Determination of Total Ammonia Excretion in Embryo, Zoea and Post-larval Stages of the Giant clawed prawn, Macrobrachium rosenbergii (De Man, 1879)
نویسنده
چکیده
Present study was conducted to determine the dry mass and the total ammonia-N (TAN) excretion in embryos, Zoea (I–XI) and post-larvae (PL) stages of Macrobrachium rosenbergii at 1, 5, and 10 d after metamorphosis (PL1, PL5, and PL10). Tested animals were collected according to their developmental stages, and placed into incubation chambers (∼50 mL) for 2 hours to calculate the TAN excretion of the animal. After this period, analyses were carried out for individual TAN determination. Individual TAN excretion generally increased throughout the larval development and varied from 0.05 ± 0.021 μg TAN/individual/h in embryo to 0.945 ± 0.232 μg TAN/individual/h in PL10. There was no significant difference between embryo and zoea (P >0.05), whereas in post larvae (PL1, PL5, and PL10) differed (P <0.05) from each other. Higher increments in individual ammoniaN excretion were observed between Zoea V to Zoea X and PL1–PL10. The lowest value was found in embryo (0.05 ± 0.021 μg TAN/mg DM/h) and the maximum values in Zoea-V and PL10 (0.22 ± 0.34 and 0.64 ± 0.63 μg TAN/mg DM/h, respectively). Results indicate that metabolic rate is proportional to the body mass in M. rosenbergii, during early life stages. Variations in ammonia excretion during this phase may be associated mainly with body size. Based on the results of the present study may be useful in developing and optimizing rearing techniques of M. rosenbergii, such as rearing tank size, stocking density and culturing methods. © 2013 Universal Research Publications. All rights reserved
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